Things You Need To Know While Using Fiber photometry
Whenever you wish to have fiber photometry, a population of neurons can be recorded using a single fiber light source without any additionally of spatial complication information. Fiber photometry cannot be used unless the fluorescent sensors such as dopamine and calcium indicators are first shown at the area of interest. Afterwards, the optical fiber will be used to detect the resulting fluorescence and also to excite the sensor.
However, when used in animals which are moving freely, it can be a problem using a ling optical capable to the head of the animal. As a result of having a long rope, there could restriction of the animal to interact while at the same time impend it to movements to area which has got shade or exercise wheels. Additionally, if chosen to be used together with video tracking systems, these capabilities can lead to objects. Meanwhile, sometimes the cable might continue to swing whereas the animal has stopped to move, and thus it might be difficult for the software to detect freezing behavior. However, if you want to end all these problems, choose to have the components placed on a head stage. Choose to know from this article, some benefits ethic results from using this technique.
Choose to use fiber photometry because it results to reduced weight on the head of the animal, as electrons wot be mounted on its head. Additionally, there is negligible generation in the tissues inside the head of an animal, as there are no electrons to be installed. Fiber photometry is much affordable because its simple set up will consist of fiber and fiber amount, simple photoelectrons, bandpass filters as well as a detector, of which all these are inexpensive photodiode. Increasingly, choose to have a fiber photometry, as there are possibilities of rapid rate acquisition which is facilitated by the analogue to digital conversion of photodiodes.
More so, choose to have fiber photometry because its photodiodes have a broader sensitivity. High sensitivity is brought about by picking up of messages from a large amount thus detecting even the low activity levels of photons. Increasingly, it is possible to achieve various geometrical and optical qualities as the fiber can be customized in several ways such as tethering, painting or being etched. Increasingly, the method is invasiveness because here because the number of fibers used is less than all the other way which uses implants. Also, thinner fibers can as well be used, but this would eventually lead to compromise when recording, or else lead to low level of signals.